Effects of 5,6-dimethylbenzimidazole and cobalt supplementation in high-concentrate diets on rumen fermentation and microorganisms and ruminal metabolome in sheep
Supplementing high-concentrate diets with 5,6-DMB (100 mg/d) and cobalt (0.5 mg/kg) increased ruminal pH, ammonia nitrogen concentration, and propionate concentration to a certain extent, reduced lactate concentration and lactate dehydrogenase activity, regulated ruminal microbial community structure (Quinella and Succinivibrio), and improved rumen metabolite levels (L-malate, pyruvate, and acetate concentrations). Conclusively, these findings suggest that supplementing high-concentrate diets with 5,6-DMB and cobalt may promote vitamin B12 synthesis and improve energy utilization efficiency.
Authors
- Zhiqiang Cheng (ORCID: https://orcid.org/0000-0003-3246-1322)
- Mengfei Li (ORCID: https://orcid.org/0000-0003-3251-624X)
- Zihao Xu (ORCID: https://orcid.org/0000-0002-7821-2789)
- Kailun Yang (ORCID: https://orcid.org/0000-0002-3192-6231)
- Changjiang Zang (ORCID: https://orcid.org/0000-0002-9862-2013)
- Fengming Li (ORCID: https://orcid.org/0000-0003-2263-1957)
- Rui Zhang (ORCID: https://orcid.org/0000-0003-2572-6142)
- Xiaobin Li (ORCID: https://orcid.org/0000-0002-8936-7161)
- Long Sun (ORCID: https://orcid.org/0000-0002-2555-7556)
- Yiling Jiao
- Yaqi Qiu
- Wentao Liu (ORCID: https://orcid.org/0009-0008-9257-8155)
Institutions
- Xinjiang Agricultural University (CN)
- Xinjiang Entry-Exit Inspection and Quarantine Bureau (CN)
- Karamay Central Hospital (CN)
Publication Details
- Journal
- BMC Veterinary Research
- Published
- 2025-12-02
- DOI
- https://doi.org/10.1186/s12917-025-05150-8
- Citations
- 2
- Primary Topic
- Ruminant Nutrition and Digestive Physiology
- Type
- article
- Field-Weighted Citation Impact
- 3.81