Enhancement of sorghum biomass hydrolysis by addition of amylases to an ionic liquid-based deconstruction process

We found that amylases are compatible with cholinium-based ionic liquids and act synergistically with other saccharolytic enzymes to deconstruct sorghum biomass. By using mixtures of sorghum grain (high starch content) and stover (low starch content), we show that amylases can produce up to 4-fold higher glucose yields from biomass with a higher starch content but even samples containing only stover can benefit from amylase addition. Total monomeric sugar concentrations of 70 and 153 g/L were obtained from stover or grain biomass, respectively, when performing a reaction with 20% solids in presence of amylases. This study presents new possibilities for process intensification during the deconstruction of starch-containing bioenergy feedstocks.

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Publication Details

Journal
BMC Research Notes
Published
2025-12-02
DOI
https://doi.org/10.1186/s13104-025-07469-9
Primary Topic
Biofuel production and bioconversion
Type
article
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article

Enhancement of sorghum biomass hydrolysis by addition of amylases to an ionic liquid-based deconstruction process

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Enhancement of sorghum biomass hydrolysis by addition of amylases to an ionic liquid-based deconstruction process

Paul Wolski, Corinne D. Scown, Henrik Vibe Scheller, Jackie Atim, Edward E. K. Baidoo, Alberto Rodriguez, Hemant Choudhary, Venkataramana R. Pidatala, John M. Gladden, Blake A. Simmons, Md Tahmid Islam, Daniel H. Putnam, Robert B. Hutmacher
article en

Abstract

We found that amylases are compatible with cholinium-based ionic liquids and act synergistically with other saccharolytic enzymes to deconstruct sorghum biomass. By using mixtures of sorghum grain (high starch content) and stover (low starch content), we show that amylases can produce up to 4-fold higher glucose yields from biomass with a higher starch content but even samples containing only stover can benefit from amylase addition. Total monomeric sugar concentrations of 70 and 153 g/L were obtained from stover or grain biomass, respectively, when performing a reaction with 20% solids in presence of amylases. This study presents new possibilities for process intensification during the deconstruction of starch-containing bioenergy feedstocks.

BMC Research NotesVol. 19(1)
Lawrence Berkeley National Laboratory (US), Sandia National Laboratories California (US), University of California Division of Agriculture and Natural Resources (US), Joint BioEnergy Institute (US), University of California, Davis (US), University of California, Berkeley (US)
Openalex Percentile: Top 63%
Biofuel production and bioconversion
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