Structure and biological activity of a novel polysaccharide from marine <i>Cladosporium velox</i> SCAU272

A new kind of purified polysaccharide (CVP) was obtained from the marine fungus strain of <i>Cladosporium velox</i> SCAU272, which was obtained from the South Oceanographic Institute of China. Through the determination of the physical and chemical properties and structural characteristics of CVP, it was found that its molecular weight is 2.726 kDa and its monosaccharide composition consists exclusively of glucose. The main framework consists of six glucose units linked through a residue-based approach, and its structure is as follows: α-D-Glcp-(1→4)-α-D-Glcp-(1→3,4)-α-D-Glcp-(1→4,6)-α-D-Glcp-(1→[4)-α-D-Glcp-(1]₇ as the main chain and α-D-Glcp-(1→3)-β-D-Glcp、α-D-Glcp-(1→ 2)-β-D-Glcp as two side chains. We performed CCK-8 and single cell colony formation experiments on macrophage 264.7, demonstrating that CVP is non-toxic and promotes cell proliferation.