Generation and characterization of an ovine cell line derived from peripheral blood and its potential use in the study of livestock and zoonotic viral infections
In this work, we isolated MSCs from ovine peripheral blood and established a continuous ovine cell line. The thorough characterization conducted as well as the osteogenic and neuronal differentiation capacity of these cells confirmed that the ovine cell line had a mesenchymal stromal origin. Interestingly, the newly established ovine cell line was permissive to all viruses studied in this work, suggesting its potential as a valuable tool for isolating and characterizing viruses and studying virus-host interactions.
Authors
- Eva Calvo-Pinilla (ORCID: https://orcid.org/0000-0002-4667-4081)
- Luís Jiménez-Cabello (ORCID: https://orcid.org/0000-0001-8085-5823)
- Silvia Rodríguez-Fernández (ORCID: https://orcid.org/0000-0002-0669-7852)
- Javier Ortego (ORCID: https://orcid.org/0000-0002-4275-7277)
- Sandra Moreno (ORCID: https://orcid.org/0000-0002-3548-037X)
- Alejandro Brun (ORCID: https://orcid.org/0000-0001-7865-538X)
- Gema Lorenzo (ORCID: https://orcid.org/0000-0003-1869-9051)
- Aitor Nogales (ORCID: https://orcid.org/0000-0002-2424-7900)
- Clara Sanjurjo‐Rodríguez (ORCID: https://orcid.org/0000-0003-2702-7804)
- Silvia Díaz‐Prado (ORCID: https://orcid.org/0000-0001-9776-2180)
- A Marín-López (ORCID: https://orcid.org/0000-0002-1010-4829)
Institutions
- Consejo Superior de Investigaciones Científicas (ES)
- Yale University (US)
- Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (ES)
- Centro de Investigación Biomédica en Red (ES)
- Instituto de Investigación Biomédica de A Coruña (ES)
Publication Details
- Journal
- Virology Journal
- Published
- 2025-12-02
- DOI
- https://doi.org/10.1186/s12985-025-03009-w
- Primary Topic
- Virus-based gene therapy research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Xunta de Galicia
- Consejo Superior de Investigaciones Científicas
- Instituto de Salud Carlos III