Regulation of granin neuropeptide gene expression in human brain during development

The granin gene family of neuropeptides functions as peptide neurotransmitters in the brain for the regulation of neural functions that regulate behaviors. Granins are involved in regulating cognition, memory, depression, aggression, stress, energy expenditure, inflammation, and related. Development of the human brain involves formation of synapses and their spectrum of neurotransmitters to establish neural connections that are required for brain functions. Therefore, the goal of this study was to analyze the gene expression profiles of the granin neurotransmitter genes during human brain development at prenatal, infancy, childhood, adolescence, and adult stages. Granin gene expression in brain development was assessed by quantitative RNA sequencing data from the Allen Human Brain Atlas resource. <i>VGF</i> (neurosecretory protein VGF) expression was significantly increased during development during the prenatal to childhood through adult stages in the anterior cingulate cortex, dorsolateral prefrontal cortex, inferolateral temporal cortex, orbital frontal cortex, posteroventral parietal cortex, primary somatosensory cortex, and primary visual cortex regions. <i>SCG2</i> (secretogranin 2) expression was also significantly increased from prenatal to infancy through adult stages in anterior cingulate cortex, dorsolateral prefrontal cortex, inferolateral temporal cortex, orbital frontal cortex, posterior superior temporal cortex, posteroventral parietal cortex, primary somatosensory cortex, and primary visual cortex. A modest number of brain regions showed increased <i>CHGA</i>, <i>CHGB</i>, and <i>SCG3</i> expression in the postnatal periods compared to the prenatal periods. Further, the <i>SCG5, PCSK1N, and GNAS</i> genes displayed minimal changes throughout development. Overall, these results demonstrate developmental upregulation of <i>VGF</i> and <i>SCG2</i> genes, with lesser upregulation of <i>CHGA</i>, <i>CHGB</i>, and <i>SCG3</i> genes, and almost no changes in <i>SCG5</i>, <i>PCSK1N</i>, and <i>GNAS</i> genes during development. These findings illustrate the differential regulation of granin genes during human brain development.