Investigation of the Effects of Prebiotics, Probiotics, and Eubiotics on Performance and Intestinal Histomorphometry in Broiler Chickens

This study was designed to investigate the effects of dietary supplementation with Prebiotics, Probiotics, and Eubiotics as alternatives to antimicrobial growth promoters (AGPs) on growth performance and intestinal histomorphometry in Ross 308 broiler chickens. A total of 200 broiler chicks were randomly allocated to five dietary treatments: basal diet (negative control), AGPs (positive control), Prebiotics, Probiotics, and Eubiotics. Key performance indicators such as weight gain (WG), feed intake (FI), feed conversion ratio (FCR), and mortality were recorded over a 42-day trial period, alongside the intestinal histomorphometries. Raw data were collected and analyzed using one-way analysis of variance (ANOVA), followed by the Least Significant Difference (LSD) post-hoc test for pairwise comparisons. Results indicated that broilers that received Prebiotics, Probiotics, and Eubiotics supplementation exhibited significantly improved WG and FCR compared to the negative control group (p < 0.05). These improvements were comparable to those observed in the AGP-supplemented group, however, no significant differences were observed between them (p >0.05). Feed intake was significantly influenced (p<0.05) across all supplemented groups, while FCR was was significantly affected during the first five weeks. Histomorphometric analysis revealed significantly enhanced villus height and goblet cell density in the Probiotics and Eubiotics supplemented groups. Mortality rates did not differ significantly among the treatment groups. Generally, the findings support the use of Prebiotics, Probiotics, and Eubiotics as effective and safer alternatives to AGPs in Ross 308 broiler production.