The effect of lactic acid bacteria as probiotics isolated from different sources on some parameters of the immune system of Wistar albino rats

The current study aimed to determine the altered immune indicators resulting from the use of lactic acid bacteria as probiotics, isolated from various sources. In this experiment, a sample of twelve-month-old male Wistar albino rats was taken with an average weight of 204.67 grams and a standard deviation of 8.04 grams. Three mice were randomly assigned to each of the four experimental groups for a period of one month. The groups were placed in different types of water, with the first group receiving plain water (control group) and the second and third groups receiving plain water mixed with sedimentary cells of lactic acid bacteria (LAB) isolated from the excreta of breastfed children. The fourth is on plain water mixed with lactic acid bacteria cells isolated from the excreta of bottle-fed infants. One month after the experiment began, blood samples were collected and subjected to six different immunological tests (IL-2, IL-8, TNF-𝛼, TNF-𝜅, ADP, and LEP) using ELISA kits. Both groups of natural feeders respond better than the other two groups, control and formula-fed. The mean optical density for the natural feeding groups is significantly different between the two natural-fed groups as well as between them and the other two groups of formula-fed and control. LEP and IL-8 recorded the highest optical densities among all others, which indicates that these factors mostly respond to the lactic acid bacteria (LAB) isolated from the excreta of breastfed children.