Late-stage peptide modification with salicylaldehyde tag enhances affinity for nuclear factor-kappa B essential modulator
The late-stage peptide functionalization with salicylaldehyde (SA) tags is described here as a versatile design of potential Lys-engaging, reversible-covalent ligands. This approach was applied to a known binder for NEMO, a protein involved in the activation of the pro-inflammatory transcription factor NFκB. Fluorescence anisotropy screening led to the identification of SA-tagged peptides with higher affinity than the wild-type sequence.
Authors
- Laura Belvisi (ORCID: https://orcid.org/0000-0002-3593-2970)
- Sara Pellegrino (ORCID: https://orcid.org/0000-0002-2325-3583)
- Alberto Dal Corso (ORCID: https://orcid.org/0000-0003-4437-8307)
- Damiano Rondelli (ORCID: https://orcid.org/0000-0001-5243-401X)
- Fulvio Uggeri
- Luca Pignataro (ORCID: https://orcid.org/0000-0002-7200-9720)
- Mattia Mason (ORCID: https://orcid.org/0000-0003-3320-8978)
- Kaliroi Peqini (ORCID: https://orcid.org/0009-0004-7053-2456)
- Sara Sattin (ORCID: https://orcid.org/0000-0002-5558-3915)
- Edoardo Scarpa (ORCID: https://orcid.org/0000-0002-5886-6929)
- Raffaella Bucci (ORCID: https://orcid.org/0000-0002-5465-9447)
Institutions
- University of Milan (IT)
- Istituto Nazionale Genetica Molecolare (IT)
Publication Details
- Journal
- RSC Chemical Biology
- Published
- 2025-12-01
- DOI
- https://doi.org/10.1039/d5cb00282f
- Primary Topic
- NF-κB Signaling Pathways
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Università degli Studi di Milano
- European Commission
- Fondazione Cariplo