Genetic Diversity of Robinia pseudoacacia L. Populations from North-Western Romania Revealed by ISSR Markers

This study aims to evaluate the genetic diversity of the species Robinia pseudoacacia L. using several populations selected from places located in the Crișana Region. The first six ISSRs tested gave distinct bands, with a total of 59 loci, of which 45 were polymorphic (63.85%). The percentage of polymorphic loci varied within populations from 33.33% to 100%. The average number of observed alleles (Na) is 1.7627 and the average effective number of alleles (Ne) is 1.4926, indicating that the effective variability is lower than the observed variability. For the Nei (h) gene diversity index, we recorded an average value of 0.2795, and for allelic entropy, the average value of the Shannon index (I) was 0.4137. The study finds a significant differentiation between populations, with a Gst coefficient value of 0.43 indicating that 43% of the variability is due to interpopulation differences.