Molecular mechanism of PINK1 regulation by the Hsp90 machinery
Hundreds of human kinases, including PINK1-a protein kinase associated with familial Parkinson's disease-are regulated by Hsp90 and its cochaperones. While previous studies have elucidated the mechanism of kinase loading into the Hsp90 machinery, the subsequent regulation of kinases by Hsp90 and its cochaperones remains poorly understood. In this study, using complexes obtained through PINK1 pulldown, we determine the cryo-EM structures of the human Hsp90-Cdc37-PINK1 complex at 2.84 Å, Hsp90-FKBP51-PINK1 at approximately 6 Å, and Hsp90- PINK1 at 2.98 Å. These structures, along with the bound nucleotide in the Hsp90 dimers of the three complexes, provide insights into the Hsp90 chaperone machinery for kinases and elucidate the molecular mechanisms governing cytosolic PINK1 regulation.
Authors
- Jiayue Su (ORCID: https://orcid.org/0000-0002-1109-7659)
- Shan Sun (ORCID: https://orcid.org/0000-0002-9807-626X)
- Sen‐Fang Sui (ORCID: https://orcid.org/0000-0001-5253-2592)
- Ziyi Wang (ORCID: https://orcid.org/0000-0002-8619-711X)
- Kunrong Mei (ORCID: https://orcid.org/0000-0001-7827-2944)
- Tao Liu (ORCID: https://orcid.org/0000-0002-2496-5611)
- Huifang Xiong
- Xuyang Tian (ORCID: https://orcid.org/0009-0009-9605-6626)
Institutions
- Tianjin University of Science and Technology (CN)
- Tianjin University (CN)
- Southern University of Science and Technology (CN)
- Tsinghua University (CN)
Publication Details
- Journal
- Nature Communications
- Published
- 2025-12-01
- DOI
- https://doi.org/10.1038/s41467-025-65859-w
- Citations
- 1
- Primary Topic
- Heat shock proteins research
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- National Natural Science Foundation of China
- Tsinghua University
- Nankai University
- Young Elite Scientists Sponsorship Program by Tianjin