A Thin Film Micro-Extraction Based Salivary Metabolomics and Chemometric Strategy for Rapid Lung Cancer Diagnosis
The findings of this study demonstrate that the TFME approach is a reliable and efficient platform for metabolomic profiling in lung cancer. Using pre-treatment saliva samples, the method achieved a sensitivity exceeding 90% for detecting newly diagnosed histopathologically confirmed patients. Among the metabolites analyzed, proline, hypoxanthine, and phenylalanine showed strong diagnostic potential, consistent with the pathway analyses implicating purine and phenylalanine metabolism. These results underscore the potential of salivary metabolomics as a non-invasive screening alternative in the absence of validated early lung cancer biomarkers. Additionally, TFME's high-throughput capacity, cost-effectiveness, and environmental sustainability support its feasibility for routine clinical application.
Authors
- Füsun Okçu Pelit (ORCID: https://orcid.org/0000-0003-0551-664X)
- Yasemin Başbınar (ORCID: https://orcid.org/0000-0001-9439-2217)
- Levent Pelit (ORCID: https://orcid.org/0000-0001-8090-703X)
- İlknur Erbaş (ORCID: https://orcid.org/0000-0003-1214-5066)
- Kasım Ocakoğlu (ORCID: https://orcid.org/0000-0003-2807-0425)
- Tuncay Göksel (ORCID: https://orcid.org/0000-0002-6089-1840)
- Durmuş Özdemir (ORCID: https://orcid.org/0000-0003-3297-8217)
- Nazli Mert-Ozupek (ORCID: https://orcid.org/0000-0002-1627-1548)
- Özlem Göksel (ORCID: https://orcid.org/0000-0003-1121-9967)
- Esra Sakrak
- Merve Gul
Institutions
- Izmir Institute of Technology (TR)
- Izmir University (TR)
- Dokuz Eylül University (TR)
- Ege University (TR)
- Tarsus University (TR)
Publication Details
- Journal
- Thoracic research and practice
- Published
- 2025-12-01
- DOI
- https://doi.org/10.4274/thoracrespract.2025.s007
- Primary Topic
- Metabolomics and Mass Spectrometry Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Türkiye Bilimsel ve Teknolojik Araştırma Kurumu