Bioengineered Humanoid-on-Chip Platforms: Tools for Evaluating the Effects of Environmental Exposure on Human Physiological Barriers

Our advanced bioengineered models demonstrated that environmental exposures significantly compromised barrier integrity, leading to increased translocation across the tissue construct, reduced cell-organoid viability, and dysregulated expression of inflammatory cytokines and immune cell activity. The resilience of human physiological barriers can be effectively modelled using humanized bioengineering platforms that emulate the dynamic mechanical and biochemical forces present <i>in vivo</i>. The integration of organoids, epithelial-endothelial barriers, and OoC technologies that mimics different kinds of organs, holds significant promise as robust and physiologically relevant systems for studying exposure-related responses.