Limited value of Nanopore adaptive sampling in a long-read metagenomic profiling workflow of clinical sputum samples
We conclude that the utility of NAS is often limited by the characteristics of the metagenomic sample studied, and that the factors contributing to pore loss need to be resolved before ONT sequencing can be reliably applied to long-read metagenomics.
Authors
- Kristin Kremer
- Janetta Top (ORCID: https://orcid.org/0000-0002-4620-8128)
- Andrii Slyzkyi
- Richard Anthony (ORCID: https://orcid.org/0000-0002-0550-5273)
- Anita C. Schürch (ORCID: https://orcid.org/0000-0003-1894-7545)
- Noud Hermans
- Marco C. Viveen
- Weizhen Xu
- Sarah van Erp
- Dafna Eiloz
Institutions
- TuBerculosis Vaccine Initiative (NL)
- University Medical Center Utrecht (NL)
- National Institute for Public Health and the Environment (NL)
- Van Putten-Gas Energy Observatory (Netherlands) (NL)
Publication Details
- Journal
- BMC Medical Genomics
- Published
- 2025-12-01
- DOI
- https://doi.org/10.1186/s12920-025-02272-8
- Primary Topic
- Genomics and Phylogenetic Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00