Study of Prevalence of Human Papilloma Virus (HPV) Infection in Ladies Attending Mansoura University Hospitals

The majority of cervical cancer is due to the human papillomavirus (HPV). Till now, there are no published studies about prevalence of HPV infection in cases attending Mansoura University Hospitals. So, we planned to conduct this cross-sectional study to investigate HPV prevalence in normal ladies, in ladies with cervical intraepithelial neoplasia (CIN), and cervical cancer in Mansoura University Hospitals. This study included 100 ladies whether they were normal, with CIN or with cervical cancer with age >18years. Cervical scrapings were collected by a gynecologist via a cyto-brush from the ecto and endo-cervix. Detection of HPV-DNA was done by polymerase chain reaction (PCR). Results showed that 11cases were positive for HPV and 89 cases were negative. HPV infection was statistically significant higher in patients using contraceptives (82%) compared with non-contraceptive users (12 %). Age of first marriage was statistically significantly lower among HPV positive cases. Histopathological analysis of punch biopsy showed that normal histopathology occurred in 91 cases; 87 cases (95.6 %) with negative HPV and 4 cases (4.4%) with positive HPV. All malignant cases were infected with HPV genotype 16, one of the malignant cases (50%) was infected with HPV genotype 18 as a coinfection with HPV genotype 16. So, this study suggested that HPV infection remains an important contributor to cervical pathology, particularly in relation to lifestyle and reproductive factors. The significant link between infection and early marriage, and contraceptive use, highlights the influence of behavioral and reproductive exposures on viral transmission.