Deep-freezing and decontamination strategy for a large autologous bone graft with presentation of the osteogenic potential of resident osteoblasts
This proof-of-concept study demonstrates that large autologous bone grafts can be frozen and subsequently decontaminated while retaining viable osteoblasts with high proliferative and regenerative potential. By combining a unique and successful clinical case with controlled experimental analyses, the study offers translational relevance, and the findings on osteoblast viability after freezing are promising.
Authors
- Slaven Babić (ORCID: https://orcid.org/0000-0002-5144-3739)
- Jelena Martinčić (ORCID: https://orcid.org/0000-0001-5834-0938)
- Denis Polančec
- Tiha Vučemilo
- Stanka Misir Šitum
- Dejan Blažević (ORCID: https://orcid.org/0000-0003-2908-2508)
- Marija Skoko
- Patricija Sesar
- Inga Marijanović (ORCID: https://orcid.org/0000-0001-7321-2192)
- Maja Ledinski (ORCID: https://orcid.org/0000-0002-1655-648X)
- Lucija Zenić (ORCID: https://orcid.org/0000-0001-7073-7057)
- Kruno Vukušić (ORCID: https://orcid.org/0000-0002-4867-6080)
- Ivana Vrgoč Zimić
- Srećko Sabalić (ORCID: https://orcid.org/0000-0003-0070-5206)
- Marija Zekušić (ORCID: https://orcid.org/0000-0002-7068-0617)
- Marina Bujić Mihica
- Snježana Ramić
- Iva M. Tolić (ORCID: https://orcid.org/0000-0003-1305-7922)
- Ivanka Batarilo
- Jure Pavešić
- M.A. Lloret Dura
- Dinko Vidović
- Kristina Crkvenac Gornik
- Zlata A. Sošić
Institutions
- University Hospital Centre Zagreb (HR)
- University of Rijeka (HR)
- University of Zagreb (HR)
- Croatian Institute of Transfusion Medicine (HR)
- University of Applied Health Sciences (HR)
- Catholic University of Croatia (HR)
- Ruđer Bošković Institute (HR)
- Sisters of Charity Hospital (HR)
- University of Turin (IT)
- University of Split (HR)
Publication Details
- Journal
- Stem Cell Research & Therapy
- Published
- 2025-12-01
- DOI
- https://doi.org/10.1186/s13287-025-04839-9
- Primary Topic
- Bone Tissue Engineering Materials
- Type
- article
- Field-Weighted Citation Impact
- 0.00