A simple and standardized method supports efficient derivation of clinical-grade human embryonic stem cells under feeder- and xeno-free conditions
Our study offers a robust, standardized, and simple method for deriving clinical-grade hESCs. Efficient derivation, propagation and banking of hESC lines from frozen-thawed embryos would offer a valuable cell source for advancing regenerative medicine, disease modeling, and drug development.
Authors
- Jintao Cun
- Jinyue Shi
- Jian Xu (ORCID: https://orcid.org/0000-0002-8162-889X)
- Yuansong Yu (ORCID: https://orcid.org/0000-0002-0382-8298)
- Yuanyuan Du (ORCID: https://orcid.org/0000-0002-8293-328X)
- Shenqiang Hu (ORCID: https://orcid.org/0000-0002-9256-8351)
- Hongkun Wang (ORCID: https://orcid.org/0009-0001-3199-5448)
- Xianlang Xiong
- Zhen Liang (ORCID: https://orcid.org/0000-0002-6272-3453)
- Huicheng Chen (ORCID: https://orcid.org/0000-0001-9156-3391)
- Qianqian Liu (ORCID: https://orcid.org/0000-0003-3325-5942)
- Qiong Zhang (ORCID: https://orcid.org/0000-0001-7930-7643)
- Hao Wang (ORCID: https://orcid.org/0000-0001-7661-3688)
- Fangyuan Sun
- Luxi Jiang
- Jingxin Wang (ORCID: https://orcid.org/0000-0002-7562-0269)
Institutions
- Yunnan University (CN)
- Chinese Academy of Sciences (CN)
- Sir Run Run Shaw Hospital (CN)
- Yunnan Maternal and Child Health (CN)
- Hangzhou DAC Biotech (China) (CN)
- Hangzhou Medical College (CN)
- Zhejiang University (CN)
Publication Details
- Journal
- Stem Cell Research & Therapy
- Published
- 2025-12-01
- DOI
- https://doi.org/10.1186/s13287-025-04831-3
- Primary Topic
- Pluripotent Stem Cells Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00