A novel reversed phase high performance liquid chromatography approach for stability-indicating simultaneous analysis of brimonidine tartrate and timolol maleate

The validated RP-HPLC method proved accurate, precise, sensitive, and stability-indicating for the simultaneous determination of Brimonidine Tartrate and Timolol Maleate. Its moderate GAC performance supports a balance between analytical rigor and sustainability. These findings establish the method as suitable for routine quality control and stability testing of ophthalmic formulations containing both drugs.