A 4NsL chromosome segment from Leymus mollis confers stripe rust and Fusarium head blight resistance in wheat
Leymus mollis (Trin.) Pilg. (2n = 4x = 28, NsNsXmXm) serves as a tertiary genetic pool for wheat improvement, harboring valuable traits such as disease resistance and stress tolerance. To harness this potential, we characterized two novel wheat-L. mollis translocation lines, M892 and M956, derived from a cross between common wheat and a 4Ns disomic addition line. Phenotypic evaluation revealed that M956 displays high resistance to both stripe rust and Fusarium head blight, while M892 is susceptible; both lines exhibit enhanced grain weight, with M892 additionally showing a dwarf phenotype. Cytogenetic analyses identified M892 as a T5DL-4NsL-5DL·5DS insertional translocation line and M956 as a T4NsL-5DL·5DS terminal translocation line. Genetic mapping localized the stripe rust resistance to a critical 98 Mb region on chromosome 4NsL, and comparative transcriptomics pinpointed 47 candidate resistance genes uniquely upregulated in M956. Furthermore, we developed eight 4NsL-specific molecular markers, providing a practical tool for the marker-assisted utilization of these resistance genes in wheat breeding programs.
Authors
- Xin Du (ORCID: https://orcid.org/0000-0002-6907-3609)
- Pingchuan Deng (ORCID: https://orcid.org/0000-0003-1617-3726)
- Jixin Zhao (ORCID: https://orcid.org/0000-0002-1035-8810)
- Yanzhen Wang (ORCID: https://orcid.org/0000-0002-8309-4828)
- Tingdong Li (ORCID: https://orcid.org/0000-0003-0685-2549)
- Wei Ren (ORCID: https://orcid.org/0000-0001-9749-0699)
- Chunhuan Chen (ORCID: https://orcid.org/0000-0002-4664-7227)
- Xinlun Liu (ORCID: https://orcid.org/0009-0001-0869-4372)
- Wanquan Ji (ORCID: https://orcid.org/0000-0003-0663-4568)
- Dongdong Ge (ORCID: https://orcid.org/0009-0002-4057-3703)
- Changyou Wang (ORCID: https://orcid.org/0000-0002-8797-0506)
- Tingting Li (ORCID: https://orcid.org/0000-0002-6587-8645)
- Yanlong Jin (ORCID: https://orcid.org/0000-0002-0047-7816)
- Xiaomin Xu (ORCID: https://orcid.org/0000-0003-4167-2404)
- Xiaoqi Zhao (ORCID: https://orcid.org/0000-0003-0441-0093)
Institutions
- Shihezi University (CN)
- Shanxi Agricultural University (CN)
- North West Agriculture and Forestry University (CN)
- Anhui Academy of Agricultural Sciences (CN)
- Yangzhou Vocational University (CN)
Publication Details
- Journal
- Frontiers in Plant Science
- Published
- 2025-12-01
- DOI
- https://doi.org/10.3389/fpls.2025.1711424
- Primary Topic
- Wheat and Barley Genetics and Pathology
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- Natural Science Basic Research Program of Shaanxi Province