3D dynamic magnetic microfluidic chip for efficient plasma extracellular vesicle enrichment and machine learning–based multiparametric diagnosis of hepatocellular carcinoma
This study developed an integrated microfluidic platform for rapid EV isolation and established an EVlncRNA Score model, enabling highly efficient early HCC detection, even in AFP-negative cases. A multiparametric diagnostic model further improved accuracy, offering a promising tool for clinical HCC screening. This strategy presents a robust, non-invasive liquid biopsy strategy with significant potential for early HCC detection.
Authors
- Kezhen Yi (ORCID: https://orcid.org/0000-0002-4968-0946)
- Xiaodan Xi
- Jia He (ORCID: https://orcid.org/0000-0002-2338-9501)
- Si Wu (ORCID: https://orcid.org/0000-0002-6346-7359)
- Wei‐Hua Huang (ORCID: https://orcid.org/0000-0001-8951-075X)
- Junfeng Ren (ORCID: https://orcid.org/0000-0003-0655-8411)
- Menglu Gao (ORCID: https://orcid.org/0000-0002-8707-1981)
- Xin He (ORCID: https://orcid.org/0000-0001-9011-5212)
- Lili Xu (ORCID: https://orcid.org/0000-0002-6146-5130)
- Fei Long (ORCID: https://orcid.org/0000-0002-6877-4403)
- Yue Hu (ORCID: https://orcid.org/0000-0002-4648-611X)
- Wei Zhong (ORCID: https://orcid.org/0000-0003-2507-3479)
- Fubing Wang (ORCID: https://orcid.org/0000-0002-5971-2622)
- Wei Cui (ORCID: https://orcid.org/0000-0002-2049-0508)
- Min Xie (ORCID: https://orcid.org/0000-0002-7189-7156)
- Yuan Rong (ORCID: https://orcid.org/0000-0003-0675-8421)
- Xin Hu (ORCID: https://orcid.org/0000-0002-3253-6024)
- Danfei Xu (ORCID: https://orcid.org/0000-0001-9279-6883)
Institutions
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Wuhan University (CN)
- Hubei Provincial Center for Disease Control and Prevention (CN)
- Zhongnan Hospital of Wuhan University (CN)
- Wuhan Center for Disease Control and Prevention (CN)
Publication Details
- Journal
- Journal of Nanobiotechnology
- Published
- 2025-12-01
- DOI
- https://doi.org/10.1186/s12951-025-03839-4
- Primary Topic
- Extracellular vesicles in disease
- Type
- article
- Field-Weighted Citation Impact
- 0.00