Intracellular autofluorescence enables the isolation of viable, functional human muscle reserve cells with distinct Pax7 levels and stem cell states
Autofluorescence enables the isolation of distinct human MuRC subpopulations. The AF<sup>High</sup> subset contains a high proportion of Pax7<sup>High</sup> cells and shows delayed activation yet retains engraftment efficiency that is comparable to that of the AF<sup>Low</sup> subpopulation. These findings suggests that AF could be used as a biomarker to identify functionally distinct human muscle progenitor subsets while preserving their regenerative potential for future use.
Authors
- Axelle Bouche (ORCID: https://orcid.org/0009-0005-9999-5224)
- Thomas Laumonier (ORCID: https://orcid.org/0000-0002-8662-2972)
- Didier Hannouche (ORCID: https://orcid.org/0000-0003-2060-2394)
- Diego Michel
- Perrine Castets (ORCID: https://orcid.org/0000-0002-6181-4791)
Institutions
- University of Geneva (CH)
- University Hospital of Geneva (CH)
Publication Details
- Journal
- Stem Cell Research & Therapy
- Published
- 2025-12-01
- DOI
- https://doi.org/10.1186/s13287-025-04811-7
- Primary Topic
- Mesenchymal stem cell research
- Type
- article
- Field-Weighted Citation Impact
- 0.00