Fucose dependent rotavirus and norovirus require fucosidase activity for optimal replication
Rotavirus (RV) and norovirus (NoV) are enteric viruses responsible for acute gastroenteritis that require fucosylated histo-blood group antigens for infection in humans. How the interaction of these viruses with fucosylated glycans modulates infection is not well understood. Treatment of target cells with a bacterial α1,2 fucosidase enzyme reduced RV and NoV infection in vitro, but increased replication in vivo. Conversely, the fucosidase inhibitor 1-deoxyfuconojirimycin impaired viral replication in both models, highlighting the role of fucosidase activity in fucose-dependent enteric virus infection. This underscores the complexity of fucose interactions for these viruses and implicates fucosidase activity as a potential antiviral target for RV and NoV.
Authors
- Nazaret Peña-Gil (ORCID: https://orcid.org/0000-0003-3553-4582)
- Joana Rocha‐Pereira (ORCID: https://orcid.org/0000-0001-8299-3787)
- Roberto Cárcamo‐Calvo (ORCID: https://orcid.org/0000-0003-4753-7406)
- Ana Melero (ORCID: https://orcid.org/0000-0003-0126-414X)
- Roberto Gozalbo‐Rovira (ORCID: https://orcid.org/0000-0003-3427-3800)
- Jesús Rodríguez‐Díaz (ORCID: https://orcid.org/0000-0002-9698-7684)
- Cristina Santiso-Bellón (ORCID: https://orcid.org/0000-0003-1698-5062)
- Marı́a J. Yebra (ORCID: https://orcid.org/0000-0003-4638-986X)
- Nânci Santos-Ferreira (ORCID: https://orcid.org/0000-0003-4995-5580)
- Javier Buesa (ORCID: https://orcid.org/0000-0002-3328-3428)
- Vicente Monedero (ORCID: https://orcid.org/0000-0001-7461-8047)
- Sergi López-Navarro
- Sonia Llanos-Villatoro
Institutions
- Universitat de València (ES)
- Rega Institute for Medical Research (BE)
- Instituto de Agroquímica y Tecnología de Alimentos (ES)
- INCLIVA Health Research Institute (ES)
- KU Leuven (BE)
Publication Details
- Journal
- npj Viruses
- Published
- 2025-11-29
- DOI
- https://doi.org/10.1038/s44298-025-00164-3
- Primary Topic
- Viral gastroenteritis research and epidemiology
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Fonds Wetenschappelijk Onderzoek
- KU Leuven
- Ministerio de Ciencia e Innovación
- Agencia Estatal de Investigación