Gut Microbial Modulation as Precursor for Blood Glucose Regulation Through <i>Moringa Oleifera</i> Leaves Aqueous Extract—An Experimental Study

Moringa oleifera (MO), also known as drumstick, has gained importance as a medicinal plant. In this experimental study, the prebiotic activity and glucose-balancing potential of Moringa oleifera have been investigated. Twenty-four male albino rats weighing 200-250 g were kept in the animal house. Streptozotocin-induced diabetic rats were randomly divided into four groups (n = 6 in each group). Control group (G₀) rats were given Metformin 100 mg/kg/day, treatment groups G₁, G₂, and G₃ rats were provided with MO leaves extract 100, 200, and 300 mg/kg/day, respectively, with a standard basal diet, and were monitored twice a week for 21 days. The results of stool analysis showed the growth of Lactobacillus, which is evident by increased count of 10⁴ CFU Lactobacillus in groups G₂ and G₃ compared to the control group 10³ CFU Lactobacillus, while higher 10⁵ CFU Lactobacillus were observed in group G₁ treated with 100 mg/kg/day of MO leaves extract. Anti-hyperglycemic results are more evident on a dose of 100 mg/kg/day from 152.50 ± 7.7 to 119 ± 7.07 (p value = 0.010) compared to other doses (p > 0.05). This study's results conclude that Moringa oleifera has promising prebiotic and anti-hyperglycemic properties and need further experimentation for a longer duration to establish the effective dose of Moringa oleifera.