A Liquid Chromatography–Mass Spectrometry-Based Approach for Analyzing Metabolites from <i>S. aureus</i>

Source: Samuels, D. J., et al. A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus. J. Vis. Exp. (2017)This video demonstrates the use of liquid chromatography–mass spectrometry to identify and quantify intracellular metabolites from S. aureus. It outlines the steps involved in metabolite extraction, peptide normalization, liquid chromatography, and mass spectrometric analysis.

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Publication Details

Journal
Journal of Visualized Experiments
Published
2025-11-28
DOI
https://doi.org/10.3791/24305
Primary Topic
Metabolomics and Mass Spectrometry Studies
Type
article
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article

A Liquid Chromatography–Mass Spectrometry-Based Approach for Analyzing Metabolites from S. aureus

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Journal of Visualized Experiments
Metabolomics and Mass Spectrometry Studies
article

A Liquid Chromatography–Mass Spectrometry-Based Approach for Analyzing Metabolites from S. aureus

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article en

Abstract

Source: Samuels, D. J., et al. A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus. J. Vis. Exp. (2017)This video demonstrates the use of liquid chromatography–mass spectrometry to identify and quantify intracellular metabolites from S. aureus. It outlines the steps involved in metabolite extraction, peptide normalization, liquid chromatography, and mass spectrometric analysis.

Journal of Visualized Experiments
Openalex Percentile: Top 66%
Metabolomics and Mass Spectrometry Studies
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A Liquid Chromatography–Mass Spectrometry-Based Approach for Analyzing Metabolites from <i>S. aureus</i> — TODO TODO · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS