Fluorescently Tagged Repressor Protein-Mediated Replication Blockage in <i>Escherichia coli</i>

Source: Mettrick, K. A., Lawrence, et al., Inducing a Site-Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System. J. Vis. Exp. (2016)This video demonstrates site-specific replication fork blockage in Escherichia coli using arabinose-induced expression of yellow fluorescent protein-tagged TetR that binds chromosomal tetO arrays.

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Publication Details

Journal
Journal of Visualized Experiments
Published
2025-11-28
DOI
https://doi.org/10.3791/24250
Primary Topic
Bacterial Genetics and Biotechnology
Type
article
Field-Weighted Citation Impact
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article

Fluorescently Tagged Repressor Protein-Mediated Replication Blockage in Escherichia coli

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Journal of Visualized Experiments
Bacterial Genetics and Biotechnology
article

Fluorescently Tagged Repressor Protein-Mediated Replication Blockage in Escherichia coli

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article en

Abstract

Source: Mettrick, K. A., Lawrence, et al., Inducing a Site-Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System. J. Vis. Exp. (2016)This video demonstrates site-specific replication fork blockage in Escherichia coli using arabinose-induced expression of yellow fluorescent protein-tagged TetR that binds chromosomal tetO arrays.

Journal of Visualized Experiments
Openalex Percentile: Top 61%
Bacterial Genetics and Biotechnology
DOISourceOpenAlex
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Fluorescently Tagged Repressor Protein-Mediated Replication Blockage in <i>Escherichia coli</i> — TODO TODO · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS