Gel Electrophoresis and Northern Blotting to Detect tRNA Charging Levels in <i>E. coli</i>

Source: Stenum, T. S., et al. Quantification of the Abundance and Charging Levels of Transfer RNAs in Escherichia coli. J. Vis. Exp. (2017)This video demonstrates the use of gel electrophoresis and northern blotting to detect and compare the charging levels of transfer RNAs in E. coli by separating aminoacylated and deacylated forms.