Soft Agar Overlay Assay for Detecting Bacteriocin-Mediated Killing in <i>Pseudomonas syringae</i>

Source: Hockett, K. L., and Baltrus, D. A. Use of the Soft-agar Overlay Technique to Screen for Bacterially Produced Inhibitory Compounds. J. Vis. Exp. (2017)This video demonstrates the procedure for detecting bacteriocin-mediated killing by spotting supernatant from an antibiotic-induced donor strain onto a soft agar overlay containing a test strain. Clear zones formed after incubation indicate bacterial inhibition.

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Publication Details

Journal
Journal of Visualized Experiments
Published
2025-11-28
DOI
https://doi.org/10.3791/24283
Primary Topic
Bacterial biofilms and quorum sensing
Type
article
Field-Weighted Citation Impact
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article

Soft Agar Overlay Assay for Detecting Bacteriocin-Mediated Killing in Pseudomonas syringae

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Journal of Visualized Experiments
Bacterial biofilms and quorum sensing
article

Soft Agar Overlay Assay for Detecting Bacteriocin-Mediated Killing in Pseudomonas syringae

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article en

Abstract

Source: Hockett, K. L., and Baltrus, D. A. Use of the Soft-agar Overlay Technique to Screen for Bacterially Produced Inhibitory Compounds. J. Vis. Exp. (2017)This video demonstrates the procedure for detecting bacteriocin-mediated killing by spotting supernatant from an antibiotic-induced donor strain onto a soft agar overlay containing a test strain. Clear zones formed after incubation indicate bacterial inhibition.

Journal of Visualized Experiments
Openalex Percentile: Top 66%
Bacterial biofilms and quorum sensing
DOISourceOpenAlex
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Soft Agar Overlay Assay for Detecting Bacteriocin-Mediated Killing in <i>Pseudomonas syringae</i> — TODO TODO · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS