Electroporation-Based Delivery of Fluorescent Biomolecules into Bacterial Cells

Source: Aigrain, L., et al. Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation. J. Vis. Exp. (2015)The video demonstrates the delivery of fluorescent biomolecules into electrocompetent microbial cells using electroporation. The process begins with the introduction of fluorophore-tagged DNA or proteins into a chilled cell suspension. A high-voltage pulse is then applied to create temporary membrane pores, enabling biomolecule entry into the cytoplasm. Following recovery, the cells undergo washing and filtration to remove non-internalized or surface-bound molecules. Finally, the labeled cells are mounted for fluorescence imaging to visualize intracellular components.