Preparation of <i>Myxococcus xanthus</i> Samples for Live-Cell Imaging

Source: Schumacher, D., et al. Fluorescence Live-cell Imaging of the Complete Vegetative Cell Cycle of the Slow-growing Social Bacterium Myxococcus xanthus. J. Vis. Exp. (2018)This video demonstrates the preparation and imaging of Myxococcus xanthus, a slow-growing soil bacterium. The culture is grown in a medium, applied to a coverslip with fluorescent microspheres, and overlaid with a pre-warmed agarose pad to support hydration and nutrient supply. This setup enables long-term, time-lapse live imaging of cellular processes during bacterial cell division.