Assessing the Protective Role of an Acid-Activated Chaperone in <i>E. coli</i> Under Acid Stress

Source: Dahl, J., et al. Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo. J. Vis. Exp. (2016)This video demonstrates the use of recombinant E. coli cultures to evaluate the protective effect of an acid-activated chaperone during acid stress. Bacterial growth monitoring reveals enhanced survival in chaperone-expressing cultures compared to controls, highlighting the role of chaperones in protein stabilization under stress conditions.