Inducible Expression of Histidine-Tagged Ricin Toxin A Chain in <i>E. coli</i>

Source: Becker, B., et al. A Simple Fluorescence-based Reporter Assay to Identify Cellular Components Required for Ricin Toxin A Chain (RTA) Trafficking in Yeast. J. Vis. Exp. (2017)This video demonstrates the inducible expression of His-tagged ricin toxin A chain (RTA) in Escherichia coli. It outlines the steps involved in the selective culturing of bacteria transformed with a recombinant expression plasmid, induction of His-tagged RTA expression, and preparation of the bacteria for further analysis.

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Publication Details

Journal
Journal of Visualized Experiments
Published
2025-11-28
DOI
https://doi.org/10.3791/24243
Primary Topic
Toxin Mechanisms and Immunotoxins
Type
article
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article

Inducible Expression of Histidine-Tagged Ricin Toxin A Chain in E. coli

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Journal of Visualized Experiments
Toxin Mechanisms and Immunotoxins
article

Inducible Expression of Histidine-Tagged Ricin Toxin A Chain in E. coli

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article en

Abstract

Source: Becker, B., et al. A Simple Fluorescence-based Reporter Assay to Identify Cellular Components Required for Ricin Toxin A Chain (RTA) Trafficking in Yeast. J. Vis. Exp. (2017)This video demonstrates the inducible expression of His-tagged ricin toxin A chain (RTA) in Escherichia coli. It outlines the steps involved in the selective culturing of bacteria transformed with a recombinant expression plasmid, induction of His-tagged RTA expression, and preparation of the bacteria for further analysis.

Journal of Visualized Experiments
Openalex Percentile: Top 58%
Toxin Mechanisms and Immunotoxins
DOISourceOpenAlex
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Inducible Expression of Histidine-Tagged Ricin Toxin A Chain in <i>E. coli</i> — TODO TODO · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS