High-Throughput Fluorescent Assay to Study Microbe-Worm Interactions

Source: Ali, I., et al. High-Throughput Screening of Microbial Isolates with Impact on Caenorhabditis elegans Health. J. Vis. Exp. (2022)This video demonstrates a high-throughput fluorescent assay to evaluate how gut bacteria affect stress resilience in Caenorhabditis elegans. By tracking blue fluorescence from ruptured gut granules, the assay reveals earlier death signals in worms exposed to test bacteria.

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Publication Details

Journal
Journal of Visualized Experiments
Published
2025-11-28
DOI
https://doi.org/10.3791/24240
Primary Topic
Genetics, Aging, and Longevity in Model Organisms
Type
article
Field-Weighted Citation Impact
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article

High-Throughput Fluorescent Assay to Study Microbe-Worm Interactions

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Journal of Visualized Experiments
Genetics, Aging, and Longevity in Model Organisms
article

High-Throughput Fluorescent Assay to Study Microbe-Worm Interactions

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article en

Abstract

Source: Ali, I., et al. High-Throughput Screening of Microbial Isolates with Impact on Caenorhabditis elegans Health. J. Vis. Exp. (2022)This video demonstrates a high-throughput fluorescent assay to evaluate how gut bacteria affect stress resilience in Caenorhabditis elegans. By tracking blue fluorescence from ruptured gut granules, the assay reveals earlier death signals in worms exposed to test bacteria.

Journal of Visualized Experiments
Openalex Percentile: Top 66%
Genetics, Aging, and Longevity in Model Organisms
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