Specific and visual detection of EBOV based on a one-pot RT-RAA-CRISPR/Cas12a assay
• The one-pot RT-RAA-CRISPR/Cas12a assay for EBOV has single-copy resolution within 40 min. • This assay targeting the VP40 gene has high specificity for EBOV and no cross-reaction with SUDV. • This one-pot assay is easy to operate and can effectively avoid aerosol contamination.
Authors
- Kaikai Jin (ORCID: https://orcid.org/0009-0002-4988-3986)
- Zhiyuan Gong (ORCID: https://orcid.org/0000-0002-9660-5260)
- Xuemeng Li (ORCID: https://orcid.org/0000-0003-1398-8313)
- Pei Huang (ORCID: https://orcid.org/0000-0003-2735-7758)
- Yuanyuan Li (ORCID: https://orcid.org/0000-0002-7263-936X)
- Tianyi Zhang (ORCID: https://orcid.org/0000-0002-2833-3363)
- Zengguo Cao
- Hualei Wang (ORCID: https://orcid.org/0000-0001-6961-1991)
- Haili Zhang (ORCID: https://orcid.org/0000-0001-8916-069X)
- Meihui Liu (ORCID: https://orcid.org/0000-0002-4726-8851)
- Zanheng Huang
- Yujie Bai
Institutions
- Guangdong Medical College (CN)
- Jilin University (CN)
- Chinese Academy of Sciences (CN)
- Wuhan Institute of Virology (CN)
Publication Details
- Journal
- Virologica Sinica
- Published
- 2025-11-28
- DOI
- https://doi.org/10.1016/j.virs.2025.11.011
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.00