A quantum dots dual immunochromatography strip for differential detection of high and low virulence ASFV antibodies
The concurrent prevalence of both high-virulence wild-type ASFV and low-virulence ASFVΔCD2v has increased the pressure on the prevention and control of ASFV. Based on the p54 and CD2v proteins of ASFV, a highly sensitive and specific quantum dots (QDs) dual immunochromatography strip (ICS) was developed using QDs conjugated with SPA as a tracer. The developed novel QDs dual ICS can rapidly detect ASFV antibodies and differentiate from high-virulence wild-type ASFV or low-virulence ASFVΔCD2v infections. This novel QDs dual ICS could be used for the serological differential diagnosis and epidemiology study of ASFV.
Authors
- Chuanwen Wang (ORCID: https://orcid.org/0009-0007-1970-3274)
- Yunhuan Zhao
- Huixia Fan
- Yuzhu Zuo (ORCID: https://orcid.org/0009-0000-1361-8107)
- Jing Ma
- Jinghui Fan (ORCID: https://orcid.org/0009-0004-6749-4583)
- Yan Li (ORCID: https://orcid.org/0000-0002-0752-1752)
- Shuai Zhang (ORCID: https://orcid.org/0000-0003-0094-1233)
Institutions
- Hebei Agricultural University (CN)
Publication Details
- Journal
- Microbiology Spectrum
- Published
- 2025-11-28
- DOI
- https://doi.org/10.1128/spectrum.02551-25
- Primary Topic
- Herpesvirus Infections and Treatments
- Type
- article
- Field-Weighted Citation Impact
- 0.00