Green Synthesis of Reduced Graphene Oxide Nanosheets for In-Vitro Anticancer Activity

Reduced graphene oxide nanosheets were synthesized using Asparagus officinalis (shatavari) leaves plant extract. Plant extract containing functional groups involvement in reduction of graphene oxide identified using functional group detection technique Fourier-Transform Infrared Spectroscopy (FTIR). The pure crystal structure of reduced graphene oxide (rGO) nanosheets (NSs) was confirmed using X-ray diffraction (XRD) pattern. Scanning electron microscopy (SEM) showed ultrathin graphene nanosheets morphology having thickness less than 7.5 nm. UV-Visible spectra of rGO shows absorption peak mainly at 279 nm. The elemental composition of rGO was studied by energy dispersive spectroscopy (EDS) confirms phytochemical reduction graphene oxide (GO). Specific surface area of rGO is 135 m2/g measured by Brunauer-Emmett-Teller (BET) technique. rGO NSs showed enhanced anticancer activity against human breast cancer cell line MDA-MB-231 tumour cells with 45 μg/mL. IC50 value emphasizing its role in biomedical field.