Usefulness of DNA Obtained from FFPE Tissue Sections Stained with Masson’s Trichrome in Forensic Identification: A Pilot Study

These results could be due to the large number of acids used in MT staining, which cause chemical modification and hydrolysis of DNA, affecting the success of PCR-based methods used subsequently. In conclusion, DNA obtained from MT-stained FFPE tissue sections may be highly degraded and should therefore be used with great caution in forensic settings.

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Publication Details

Journal
Genes
Published
2025-11-28
DOI
https://doi.org/10.3390/genes16121416
Primary Topic
Molecular Biology Techniques and Applications
Type
article
Field-Weighted Citation Impact
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article

Usefulness of DNA Obtained from FFPE Tissue Sections Stained with Masson’s Trichrome in Forensic Identification: A Pilot Study

Marta Ortega‐Martínez, Ivett Miranda-Maldonado, Juan Manuel Solís-Soto, Gilberto Jaramillo‐Rangel et al.
Genes
Molecular Biology Techniques and Applications
article

Usefulness of DNA Obtained from FFPE Tissue Sections Stained with Masson’s Trichrome in Forensic Identification: A Pilot Study

Marta Ortega‐Martínez, Ivett Miranda-Maldonado, Juan Manuel Solís-Soto, Gilberto Jaramillo‐Rangel, Jaime García‐Juárez, María-de-Lourdes Chávez-Briones, Adriana Ancer-Arellano
article en

Abstract

These results could be due to the large number of acids used in MT staining, which cause chemical modification and hydrolysis of DNA, affecting the success of PCR-based methods used subsequently. In conclusion, DNA obtained from MT-stained FFPE tissue sections may be highly degraded and should therefore be used with great caution in forensic settings.

GenesVol. 16(12)
Universidad Autónoma de Nuevo León (MX)
Openalex Percentile: Top 66%
Molecular Biology Techniques and Applications
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