Pyrrolidine Alkaloids from Mangrove Fungus Penicillium sp. DM27 Enhance L6 Cell Glucose Uptake
Ten previously undescribed pyrrolidine alkaloids, namely penicipyrrolidines O-X (<b>1</b>-<b>10</b>), were isolated from the mangrove-derived fungus <i>Penicillium</i> sp. DM27, along with five known compounds (<b>11</b>-<b>15</b>). Their structures were determined by comprehensive analysis of HRESIMS and NMR spectroscopic data, and the absolute configurations were established based on biosynthetic considerations and TDDFT-ECD calculations. All isolates were evaluated for their glucose uptake capacity. Notably, penicipyrrolidine P (<b>2</b>) significantly enhanced cellular glucose uptake in L6 myotubes by 3.83-fold, demonstrating activity comparable to that of metformin, whereas penicipyrrolidines Q and R (<b>3</b> and <b>4</b>) showed relatively weaker effects.
Authors
- Yuan-Zhen Liu
- Qingqing Tang (ORCID: https://orcid.org/0000-0002-5177-5573)
- Fengkai Fan
- Kui Hong (ORCID: https://orcid.org/0000-0002-0686-0947)
- Min Zhou (ORCID: https://orcid.org/0000-0001-5509-6845)
- Wen-Qing Huang (ORCID: https://orcid.org/0000-0001-8292-2102)
- Xinzhou Yang (ORCID: https://orcid.org/0000-0003-1697-2923)
- Wenting Zhang (ORCID: https://orcid.org/0000-0003-2236-7148)
- Liming He
- Kongkai Zhu (ORCID: https://orcid.org/0000-0003-2293-3568)
- You‐Sheng Cai (ORCID: https://orcid.org/0000-0001-9325-0241)
- Congkui Tian
- Philomina Panin Edjah (ORCID: https://orcid.org/0009-0003-5872-8539)
Institutions
- Minzu University of China (CN)
- Shandong University (CN)
- Wuhan University (CN)
- Minda Hospital (CN)
- Hubei University of Education (CN)
Publication Details
- Journal
- Marine Drugs
- Published
- 2025-11-27
- DOI
- https://doi.org/10.3390/md23120455
- Primary Topic
- Microbial Natural Products and Biosynthesis
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Wuhan University
- Zhongnan Hospital of Wuhan University
- National Key Research and Development Program of China