Recovery efficiency and functional characterization of T cells and NK cells from leukocyte filters
Reverse flushing of leukocyte filters with 40 mL NS enables cost-effective recovery of lymphocytes, particularly CD3<sup>+</sup>CD4<sup>+</sup> T cells. The recovered NK and T cells exhibit functional equivalence to those from those from whole blood samples, offering a low-cost and sustainable cell source for allogeneic cell therapy.
Authors
- Jinfeng Zeng (ORCID: https://orcid.org/0000-0002-7267-312X)
- S.‐X. Wang (ORCID: https://orcid.org/0000-0002-4583-2148)
- Liyan Sun (ORCID: https://orcid.org/0000-0003-0261-5300)
- Ran Li (ORCID: https://orcid.org/0000-0001-7202-9767)
- Siqi Cai (ORCID: https://orcid.org/0000-0003-1478-2202)
Institutions
- Sun Yat-sen University (CN)
- Longgang Central Hospital (CN)
- Shenzhen Blood Center (CN)
- Shenzhen Third People’s Hospital (CN)
- Eighth Affiliated Hospital of Sun Yat-sen University
Publication Details
- Journal
- Frontiers in Medicine
- Published
- 2025-11-28
- DOI
- https://doi.org/10.3389/fmed.2025.1681146
- Primary Topic
- Immune Cell Function and Interaction
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Sanming Project of Medicine in Shenzhen