Electrostatically driven fluorescent sensor for rapid detection of AChE activity and organophosphate pesticides via dual-enzyme cascade amplification
This study not only embedded the entire reaction chain [AChE-CHO-hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)] into the sensor to improve space utilization efficiency and detection efficiency, but also established a novel paradigm for enzyme spatial organization based on electrostatic complementarity, providing new insights into the rational design of nanostructured multi-enzyme sensing platforms.
Authors
- Guojing Liu
- Kunhui Sun
- Xie‐an Yu (ORCID: https://orcid.org/0000-0003-2918-7369)
- Lan Ma (ORCID: https://orcid.org/0000-0003-3458-1758)
- Ping Wang (ORCID: https://orcid.org/0000-0003-1192-2353)
- Bing Wang (ORCID: https://orcid.org/0000-0002-6549-8837)
- Xiaoyi Liu (ORCID: https://orcid.org/0009-0006-5862-2031)
- Lei Wei (ORCID: https://orcid.org/0000-0001-5140-0504)
- Meifang Li (ORCID: https://orcid.org/0000-0002-6916-9723)
- Shuhong Wang (ORCID: https://orcid.org/0000-0001-6400-5853)
- Sitong Lai
Institutions
- Tianjin University of Traditional Chinese Medicine (CN)
- Tsinghua–Berkeley Shenzhen Institute (CN)
- Shenzhen Institute for Drug Control (CN)
Publication Details
- Journal
- Frontiers in Pharmacology
- Published
- 2025-11-28
- DOI
- https://doi.org/10.3389/fphar.2025.1679948
- Primary Topic
- Electrochemical sensors and biosensors
- Type
- article
- Field-Weighted Citation Impact
- 0.00