Dual-Modality Ultrasound Imaging of SPIONs Distribution via Combined Magnetomotive and Passive Cavitation Imaging

Superparamagnetic iron oxide nanoparticles (SPIONs) have shown promise across a wide range of biomedical applications, including targeted drug delivery, magnetic hyperthermia, magnetic resonance imaging, and regenerative medicine. In the context of local tumor therapy (Magnetic Drug Targeting, MDT) SPIONs can be functionalized with chemotherapeutic agents and accumulated at tumor sites using an externally applied magnetic field. To achieve effective drug accumulation and therapeutic efficacy, precise positioning of the accumulation magnet relative to the tumor is essential. To address this need, we propose a dual-modality ultrasound imaging approach combining magnetomotive ultrasound (MMUS) and passive cavitation mapping (PCM). MMUS detects magnetically induced displacements to localize SPIONs embedded in elastic tissue, while PCM monitors cavitation emissions from circulating SPIONs under focused ultrasound exposure. In addition to detection, PCM has the potential to enable feedback-based control of cavitation exposure, allowing cavitation parameters to be kept within a safe regime. The dual imaging modality approach was validated using standard phantoms and a complex carotid bifurcation tumor flow phantom fabricated via 3D printing. Experimental results demonstrate the first coordinated spatiotemporal imaging of MMUS and PCM within the same anatomical model, resolving the key bottleneck of SPIONs monitoring in blood vessels/tissue. This demonstrates the strong potential of complementary MMUS and PCM imaging for monitoring in preclinical and clinical MDT settings.