Establishment of rapid saturation mutagenesis and screening methods for improving the neutralizing activity of monoclonal antibodies
We established a high-throughput saturation mutagenesis platform for antibody affinity modification, enhancing the anti-infection ability of the antirabies NC08 antibody through single-point and multiple-point mutants. This platform is also applicable for optimizing other mAbs in preclinical stages in terms of neutralizing activity and achieving broad-spectrum activity.
Authors
- Youchun Wang (ORCID: https://orcid.org/0000-0001-9769-5141)
- Xi Wu (ORCID: https://orcid.org/0000-0002-6180-741X)
- Yanru Shen
- Weijin Huang (ORCID: https://orcid.org/0000-0002-4246-8889)
- Lijie Wang (ORCID: https://orcid.org/0000-0001-8674-3128)
- C. He
Institutions
- Shenyang Pharmaceutical University (CN)
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Peking Union Medical College Hospital (CN)
- National Institutes for Food and Drug Control (CN)
- Beijing Wantai Biological Pharmacy (China) (CN)
Publication Details
- Journal
- Frontiers in Immunology
- Published
- 2025-11-27
- DOI
- https://doi.org/10.3389/fimmu.2025.1722831
- Primary Topic
- Monoclonal and Polyclonal Antibodies Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00