Peach Kernel Oil Modulates Lipid Metabolism Disorder in <i>ApoEa</i> CRISPR/ <i>Cas9</i> Gene‐Disrupted Zebrafish

ABSTRACT Due to today's unhealthy eating habits, such as high‐fat diets (HDs), people's bodies are in a sub‐healthy or unhealthy state, in which lipid disorders are a major cause of cardiovascular disease. Peach kernel oil (PKO), a by‐product of peaches, is rich in oleic acid and linoleic acid, but there are few studies on its biological characteristics. Zebrafish is an excellent animal model for the study of lipid metabolism. This study was designed to establish a lipid disorder model of zebrafish with an ApoEa genotype defect to investigate how PKO regulates lipid disorders. According to our findings, the ApoEa heterozygote ( Het ApoEa ) zebrafish lipid metabolism disorder model could be established by feeding with HD for 5 days. Het ApoEa zebrafish fed with HD for 5 days showed a significant increase in total cholesterol, lipid accumulation in the arterial vessels, and lipid droplet abundance in liver cells accompanied by mitochondrial damage. The role of PKO in the lipid metabolism disorder model of zebrafish revealed that PKO inhibits the accumulation of lipids and decreases the formation of neutrophils induced by HD. In the in vitro high‐fat, oxidative‐stress, and inflammatory models of vascular (HUVECs) and hepatic (HepG2) cells, PKO suppressed intracellular lipid accumulation, decreased reactive oxygen species (ROS) generation, and restored cell viability after inflammatory injury. RNA‐seq analysis results showed that PKO activates the Jak‐STAT signaling pathway by inhibiting stat1b and pkr expression. Our findings suggested that PKO, as a naturally active product, has excellent potential for use in the development of foods.