Optimization of Bioethanol from Plantain Peel Using Saccharomyces Cerevisiae

This research aimed to evaluate the potential of using plantain peels as a raw material for producing bioethanol with the help of Saccharomyces cerevisiae. The study utilized a four-factor Box-Behnken design (BBD) and response surface methodology (RSM) to optimize the fermentation conditions. The factors considered for optimization were substrate concentration (1-4 g), pH (5-7), temperature (30-45°C), and fermentation time (24-96 hours). Through this optimization process, the study found that the optimal conditions for bioethanol production were 4 g substrate concentration, pH 6, 45°C temperature, and 60 hours of fermentation time. Utilizing these optimal conditions resulted in a bioethanol yield of 5.45%. This study suggests that plantain peels can be a sustainable source for bioethanol production. Furthermore, the study validated the use of Box-Behnken design and Response Surface Methodology as reliable techniques to determine the effects of fermentation parameters and identify optimal conditions for bioethanol production.