Critical Assessment of MetaProteome Investigation 2 (CAMPI-2): multi-laboratory assessment of sample processing methods to stabilize fecal microbiome for functional analysis
CAMPI-2 allowed a robust evaluation of five different methods for preserving fecal metaproteome samples. The present investigation provides useful data for the design of metaproteomics and multi-omics studies where fecal sampling cannot be immediately followed by long-term storage at - 80 °C. Further optimization of the tested protocols is necessary to improve stabilization efficiency and control bias in the taxonomic and functional profile of the gut microbiome. Video Abstract.
Authors
- Guylaine Miotello (ORCID: https://orcid.org/0000-0002-2398-1249)
- Robert L. Hettich (ORCID: https://orcid.org/0000-0001-7708-786X)
- Dirk Benndorf (ORCID: https://orcid.org/0000-0003-4021-8525)
- Felipe Bastida (ORCID: https://orcid.org/0000-0001-9958-7099)
- Benoît J. Kunath (ORCID: https://orcid.org/0000-0002-3356-8562)
- Nico Jehmlich (ORCID: https://orcid.org/0000-0002-5638-6868)
- Lucia Grenga (ORCID: https://orcid.org/0000-0001-5560-1717)
- Maximilian Wolf (ORCID: https://orcid.org/0000-0002-8770-8399)
- Jana Seifert (ORCID: https://orcid.org/0000-0002-7690-8539)
- Kay Schallert (ORCID: https://orcid.org/0000-0002-1683-7222)
- Jean Armengaud (ORCID: https://orcid.org/0000-0003-1589-445X)
- Johan S. Sáenz (ORCID: https://orcid.org/0000-0002-7240-8561)
- Robert Heyer
- Alessandro Tanca
- Sven‐Bastiaan Haange (ORCID: https://orcid.org/0000-0003-2952-1152)
- Samantha L. Peters (ORCID: https://orcid.org/0000-0002-3755-9786)
- Simon Devos (ORCID: https://orcid.org/0000-0002-0623-433X)
- Paul Wilmes (ORCID: https://orcid.org/0000-0002-6478-2924)
- Laura De Diego (ORCID: https://orcid.org/0009-0000-8249-5083)
- Sergio Uzzau (ORCID: https://orcid.org/0000-0001-6246-2794)
- Guillermina Hernandez‐Raquet (ORCID: https://orcid.org/0000-0003-4465-4607)
- Tim Van Den Bossche (ORCID: https://orcid.org/0000-0002-5916-2587)
- Marcello Abbondio
- Maria Antonietta Deledda
Institutions
- University of Hohenheim (DE)
- Oak Ridge National Laboratory (US)
- Centre National de la Recherche Scientifique (FR)
- Helmholtz Centre for Environmental Research (DE)
- University of Sassari (IT)
- Université Fédérale de Toulouse Midi-Pyrénées (FR)
- University of Luxembourg (LU)
- Institut National des Sciences Appliquées de Toulouse (FR)
- Bielefeld University (DE)
- Commissariat à l'Énergie Atomique et aux Énergies Alternatives (FR)
- Université Paris-Saclay (FR)
- Ghent University (BE)
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- Anhalt University of Applied Sciences (DE)
- Centro de Edafología y Biología Aplicada del Segura (ES)
- Leibniz Institute for Analytical Sciences - ISAS (DE)
- CEA Paris-Saclay (FR)
- VIB-UGent Center for Medical Biotechnology (BE)
- Otto-von-Guericke-Universität Magdeburg (DE)
Publication Details
- Journal
- Microbiome
- Published
- 2025-11-27
- DOI
- https://doi.org/10.1186/s40168-025-02248-x
- Citations
- 2
- Primary Topic
- Gut microbiota and health
- Type
- article
- Field-Weighted Citation Impact
- 1.61
Funders
- European Commission
- Agence Nationale de la Recherche
- Fonds Wetenschappelijk Onderzoek
- Ministero della Salute
- Ministero dell’Istruzione, dell’Università e della Ricerca
- Vlaamse regering
- Région Occitanie Pyrénées-Méditerranée
- CHIST-ERA