Exosome-functionalized collagen-coated 3D-printed PCL scaffold for enhanced osteogenic differentiation and bone regeneration: an in vitro and in vivo study
These findings demonstrate that osteoblast-derived exosomes serve as potent bioactive modulators capable of driving osteogenic differentiation and accelerating bone regeneration. Incorporating exosomes into 3D printed PCL/Col scaffolds provides a promising biomimetic and partially cell-free approach for the treatment of critical-sized bone defects. This strategy highlights the potential of combining advanced scaffold fabrication with bioactive vesicles to improve clinical outcomes in regenerative medicine.
Authors
- Mahsa Kouhestani (ORCID: https://orcid.org/0009-0006-8835-8921)
- Abdolreza Mohamadnia (ORCID: https://orcid.org/0000-0001-9662-7462)
- Naghmeh Bahrami (ORCID: https://orcid.org/0000-0002-3575-4094)
- Sadegh Shirian
- Mohammad Bayat (ORCID: https://orcid.org/0000-0002-5235-1203)
- Mohammad Reza Karimzadeh (ORCID: https://orcid.org/0000-0003-1698-1686)
- Moosa Javdani (ORCID: https://orcid.org/0000-0003-0975-2295)
- Somayeh Ebrahimi‐Barough
- Jafar Ai
- Fatemeh Ghorbani Shemshadsara
Institutions
- Shahrekord University (IR)
- University of Minnesota (US)
- Masih Daneshvari Hospital (IR)
- Shariati Hospital (IR)
- Shahid Beheshti University (IR)
- Shahid Beheshti University of Medical Sciences (IR)
- Tehran University of Medical Sciences (IR)
Publication Details
- Journal
- Journal of Biological Engineering
- Published
- 2025-11-27
- DOI
- https://doi.org/10.1186/s13036-025-00578-w
- Primary Topic
- Extracellular vesicles in disease
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Tehran University of Medical Sciences and Health Services