Arabidopsis root lipid droplets are hubs for membrane homeostasis under heat stress, and triterpenoid synthesis and storage
Plant lipid droplets (LDs) and their associated proteins have numerous subcellular and physiological functions. While considerable progress has been made for LDs in many tissues, the function and composition of LDs in roots remain largely unexplored. We investigated the changes in the number of LDs and the lipidome in heat-stressed Arabidopsis thaliana roots. Furthermore, we isolated root LDs from the Arabidopsis mutant trigalactosyldiacylglycerol 1-1 sugar dependent 1-4 and investigated its proteome and lipidome. Heat stress led to a degradation of membrane lipids and an increase in triacylglycerols and LDs, while fatty acid steryl esters decreased, probably acting as precursors for acylated sterol glycosides. A variety of proteins were enriched in root LDs, which are thus far not described as LD proteins. Transient expression of these proteins in many cases confirmed their LD localization, for example of the triterpene biosynthetic enzymes thalianol synthase and marneral synthase. We could furthermore show that the educts and products of these enzymes are enriched in root LDs, too. We conclude that root LDs simultaneously act as a sink and source during heat stress-induced membrane remodeling. Furthermore, root LDs play a pivotal role in triterpene synthesis and storage, thereby highlighting LDs as hubs in specialized metabolism.
Authors
- Robert T. Mullen (ORCID: https://orcid.org/0000-0002-6915-7407)
- Patricia Scholz (ORCID: https://orcid.org/0000-0003-0761-9175)
- Iris Finkemeier (ORCID: https://orcid.org/0000-0002-8972-4026)
- Magdiel Sheng Satha Lim (ORCID: https://orcid.org/0000-0003-1463-6467)
- Martin Bonin (ORCID: https://orcid.org/0000-0002-6910-2174)
- Philipp William Niemeyer (ORCID: https://orcid.org/0000-0003-2002-8097)
- Peter Dörmann (ORCID: https://orcid.org/0000-0002-5845-9370)
- Lea Hembach (ORCID: https://orcid.org/0000-0002-1575-7423)
- Katharina Gutbrod (ORCID: https://orcid.org/0000-0003-4473-1414)
- Katharina Blersch (ORCID: https://orcid.org/0000-0001-7020-5587)
- Mayuko Naganawa (ORCID: https://orcid.org/0000-0002-6820-8997)
- Takashi Shimada (ORCID: https://orcid.org/0000-0001-6185-4657)
- Francine Dreier (ORCID: https://orcid.org/0009-0004-9933-7515)
- Ana Carolina Vilchez (ORCID: https://orcid.org/0000-0002-1486-4577)
- Elena Lesch (ORCID: https://orcid.org/0000-0002-3449-3929)
- Till Ischebeck (ORCID: https://orcid.org/0000-0003-0737-3822)
- Alyssa C. Clews (ORCID: https://orcid.org/0009-0009-4568-2934)
- Janis Dabisch (ORCID: https://orcid.org/0009-0008-4840-5564)
- You Wang (ORCID: https://orcid.org/0000-0001-6058-9900)
- Siqi Sun (ORCID: https://orcid.org/0000-0003-1240-1189)
- Y. Iwai
- Jürgen Eirich (ORCID: https://orcid.org/0000-0003-0963-1872)
- Lea M Preuß (ORCID: https://orcid.org/0009-0000-0946-5830)
Institutions
- Université Claude Bernard Lyon 1 (FR)
- Centre National de la Recherche Scientifique (FR)
- University of Bonn (DE)
- Chiba University (JP)
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- Institute of Plant Biology and Biotechnology (KZ)
- Matsudo Museum (JP)
- Laboratoire Reproduction et Développement des Plantes (FR)
- University of Göttingen (DE)
- University of Guelph (CA)
Publication Details
- Journal
- New Phytologist
- Published
- 2025-11-25
- DOI
- https://doi.org/10.1111/nph.70726
- Primary Topic
- Lipid metabolism and biosynthesis
- Type
- article
- Field-Weighted Citation Impact
- 0.00