From loops to caps: discriminating peptide binding to distinct G-quadruplex tetrads using 5-furyl-2′-deoxyuridine fluorescent probes
We report the use of 5-furyl-2'-deoxyuridine (5FU) as a fluorescent probe to distinguish ligand binding to distinct G-quadruplex tetrads. Site-specific incorporation of 5FU into T95-2T at the capping regions enabled discrimination of peptide-binding events <i>via</i> fluorescence turn-on, providing insights into tetrad preference and binding affinities using the 5FU-G4/RHAU peptide system.
Authors
- Christine J. Cardin (ORCID: https://orcid.org/0000-0002-2556-9995)
- Enrico Cadoni (ORCID: https://orcid.org/0000-0001-5585-7579)
- Jack Barr (ORCID: https://orcid.org/0000-0001-9819-1682)
- Tayler D. Prieto Otoya
Institutions
- Ghent University (BE)
- University of Reading (GB)
Publication Details
- Journal
- RSC Chemical Biology
- Published
- 2025-11-10
- DOI
- https://doi.org/10.1039/d5cb00247h
- Primary Topic
- DNA and Nucleic Acid Chemistry
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- European Commission
- Fonds Wetenschappelijk Onderzoek
- Biotechnology and Biological Sciences Research Council