Investigating genetic modifications to enhance L1CAM-CAR T cell migration in solid tumors in a 3D bioprinted neuroblastoma model
Our findings reveal a key limitation of murine xenograft models, which are widely used as the gold standard for preclinical CAR T cell testing. The lack of conservation between the human and murine SELPLG proteins likely accounts for the discrepancy between enhanced <i>in vitro</i> migration of SELPLG-deficient L1CAM-CAR T cells and their lack of improved efficacy in the mouse model. This underscores the need for more predictive human-relevant models to better preclinically evaluate CAR T cell function.
Authors
- Anton George Henssen (ORCID: https://orcid.org/0000-0003-1534-778X)
- Tobias Lam (ORCID: https://orcid.org/0000-0003-1404-7822)
- Silke Schwiebert
- Kathleen Anders (ORCID: https://orcid.org/0000-0002-0429-200X)
- Annette Künkele (ORCID: https://orcid.org/0000-0002-8406-5412)
- Maria Stecklum (ORCID: https://orcid.org/0009-0001-7951-5730)
- Marvin Jens (ORCID: https://orcid.org/0000-0002-6660-8743)
- Johannes H. Schulte (ORCID: https://orcid.org/0000-0003-0671-1201)
- Laura Grunewald (ORCID: https://orcid.org/0000-0002-7175-7380)
- Lena Andersch
- Angelika Eggert (ORCID: https://orcid.org/0000-0003-3476-8184)
- Lutz Kloke
- Kathy Astrahantseff
- Viola Hollek (ORCID: https://orcid.org/0000-0001-9768-5568)
- Filippos Klironomos (ORCID: https://orcid.org/0000-0003-2627-5662)
- Michael Launspach (ORCID: https://orcid.org/0000-0001-5337-5605)
- Nils Blüthgen (ORCID: https://orcid.org/0000-0002-0171-7447)
- Kerstin Haase (ORCID: https://orcid.org/0000-0002-0944-5618)
- Anika Winkler
- B. Jung (ORCID: https://orcid.org/0000-0002-3833-938X)
Institutions
- German Cancer Research Center (DE)
- Heidelberg University (DE)
- Humboldt-Universität zu Berlin (DE)
- German Cancer Society (DE)
- BioTez (Germany) (DE)
- Helios Hospital Berlin-Buch (DE)
- Berlin Heart (Germany) (DE)
Publication Details
- Journal
- Frontiers in Immunology
- Published
- 2025-11-27
- DOI
- https://doi.org/10.3389/fimmu.2025.1677361
- Primary Topic
- CAR-T cell therapy research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Deutsche Forschungsgemeinschaft