Microbial community characterization in Red Sea-derived samples using a field-deployable DNA extraction system and nanopore sequencing
The usability of different field-deployable instruments for microbial community characterization in marine-derived samples was demonstrated. Moreover, differences in beta diversity were observed when DNA was extracted in-field versus in-lab using the xTitan system, particularly for mangrove-associated samples. These results highlight the value of on-site nucleic acid extraction for enhancing the detection of microbial taxa that can be sensitive to cold storage. This study enabled the testing of the xTitan on Red Sea-derived samples, generating comprehensive information on the effects of DNA extraction systems and transportation of samples on coral and mangrove-associated microbiomes.
Authors
- Adam R. Barno (ORCID: https://orcid.org/0000-0001-9532-0012)
- Gustavo Duarte (ORCID: https://orcid.org/0000-0002-7950-3975)
- Francisca C. García (ORCID: https://orcid.org/0000-0001-5363-1015)
- Alexander Putra (ORCID: https://orcid.org/0000-0002-4864-9744)
- Diego J. Jiménez (ORCID: https://orcid.org/0000-0001-6540-8160)
- Andy Page
- Changsook Park (ORCID: https://orcid.org/0000-0003-1515-4574)
- Alexandre Soares Rosado (ORCID: https://orcid.org/0000-0001-5135-1394)
- Niketan Patel (ORCID: https://orcid.org/0000-0002-4702-7361)
- Angel Angelov (ORCID: https://orcid.org/0000-0002-9228-0817)
- Scott Tighe (ORCID: https://orcid.org/0000-0002-3988-0741)
- Stefan J. Green (ORCID: https://orcid.org/0000-0003-2781-359X)
- Tahira Jamil (ORCID: https://orcid.org/0000-0001-8754-0605)
- Georgios Miliotis (ORCID: https://orcid.org/0000-0002-0944-2206)
- Adam M. Kabza
- Raquel Silva Peixoto (ORCID: https://orcid.org/0000-0002-9536-3132)
- Lila A. Aldakheel
- Helena Villela (ORCID: https://orcid.org/0000-0002-0052-503X)
- Érika P. Santoro (ORCID: https://orcid.org/0000-0002-4962-1356)
- Season Wong (ORCID: https://orcid.org/0000-0001-5264-0286)
- Ahmed AlSaggaf
- Patrick Driguez (ORCID: https://orcid.org/0000-0001-8104-677X)
- Júnia Schultz (ORCID: https://orcid.org/0000-0002-8147-4659)
- Nicholas Kontis
- Ayman Farran
- Anna Tumeo (ORCID: https://orcid.org/0009-0007-7689-2809)
- Kasthuri Venkateswaran
Institutions
- University of Vermont (US)
- Jet Propulsion Laboratory (US)
- Ollscoil na Gaillimhe – University of Galway (IE)
- AI Biosciences (United States) (US)
- InnovaPrep (United States) (US)
- Rush University (US)
- King Abdullah University of Science and Technology (SA)
Publication Details
- Journal
- Environmental Microbiome
- Published
- 2025-11-26
- DOI
- https://doi.org/10.1186/s40793-025-00819-x
- Citations
- 1
- Primary Topic
- Microbial Community Ecology and Physiology
- Type
- article
- Field-Weighted Citation Impact
- 1.72