The Healing Potential of Platelet‐Rich Plasma: Advances in Preparation Methods, Biomedical Applications, and Emerging Challenges

Abstract Platelet‐rich plasma (PRP) is a promising therapeutic approach in regenerative medicine that has gained considerable attention due to its capability to enhance tissue repair and regeneration. Initially introduced for managing thrombocytopenia, PRP now has broad clinical applications across various biomedical fields, ranging from dermatology to neurology. Its therapeutic effects rely on delivering concentrated platelets that release critical growth factors and cytokines, thereby enhancing natural healing processes such as cellular proliferation, extracellular matrix remodeling, and angiogenesis. Despite promising clinical outcomes, PRP therapy faces challenges due to inconsistencies in preparation methods, limiting its standardization and clinical efficacy. Unlike previous studies that focus narrowly on individual applications, this comprehensive review presents a multidisciplinary overview that integrates the latest advancements in PRP preparation methods and their implications for broader clinical translation, such as orthopedics, nerve regeneration, dermatology, dentistry, and skin aging treatment. Additionally, the article addresses ongoing issues related to standardizing PRP use, ethical considerations, and identifies critical areas for future research. Through this evaluation, the review aims to provide valuable insights for clinicians and researchers, contributing to improved clinical outcomes and broader therapeutic utilization of PRP in regenerative medicine.