Evaluation of Genome-Scale Model Reconstruction Strategies for Lentilactobacillus kefiri DH5 and Deciphering Its Metabolic Network
We developed the first manually curated genome-scale model of <i>L. kefiri</i> DH5 and showed that the choice of reconstruction tool substantially affects model quality and predictive power. We also proposed an alternative glucose assimilation shunt via gluconolactone, which resolved the redox imbalance in the model and enabled representation of the heterofermentative metabolism.
Authors
- Ilya R. Akberdin (ORCID: https://orcid.org/0000-0003-0010-8620)
- Mikhail A. Kulyashov (ORCID: https://orcid.org/0000-0002-4808-0922)
- T. V. Sokolova (ORCID: https://orcid.org/0000-0003-3393-3096)
- Alexey E. Sazonov (ORCID: https://orcid.org/0000-0001-8611-5770)
- Maryam A. Esembaeva (ORCID: https://orcid.org/0000-0003-2591-459X)
Institutions
- Sirius University of Science and Technology (RU)
Publication Details
- Journal
- Metabolites
- Published
- 2025-11-26
- DOI
- https://doi.org/10.3390/metabo15120767
- Primary Topic
- Microbial Metabolic Engineering and Bioproduction
- Type
- article
- Field-Weighted Citation Impact
- 0.00